Most of refrigerators commonly use the conventional refrigeration system known as Vapor Compression Refrigeration System becoming a big issue lately due to ozone depleting substance it uses as the refrigerant. This paper will describe systematically of an experiment with the objective of constructing a refrigeration system based on thermoelectric which is reliable and compete able with the Vapor Compression Refrigeration System. The designs of this refrigeration system give emphasis on the environment that is combined with the knowledge so that the environmentally friendly technology can be applied. The performance of thermoelectric refrigerator was investigated under variation of input power (40W, 72W, and 120W) and operated in ambient temperature and cooling load of water 1000mL to investigate the characteristic of system and the coefficient of performance, COP. It is found that the COP is decrease by increasing of cooling load, Q_L. The best actual COP is 0.182 obtained when the refrigerator operated at input power 40W. The result shows that decreasing of ambient temperature affects the decreasing of cabin temperature. Thermoelectric and heat pipe refrigerator cooling system can reach cabin temperature of 10.63 °C for the power of 120Watt (8.73A, 14V) indicates effective performance work-based thermoelectric applications.