A loudspeaker-driven thermoacoustic refrigerator has been built and tested to gain understanding of its thermal performance and the cooling rate. The influence of plate thickness made of acrylic sheet was experimentally investigated by varying plate thickness of the stack, 0.15 mm, 0.5 mm and 1 mm, respectively. The experiments were conducted with various voltage input to the driver starting from setting 4 to 9 voltage peak-to-peak. The temperatures at both ends of the stack were acquired. For all variations, thermoacoustic cooling effect occurred in seconds and escalated rapidly in two minutes and became stable in ten-minute time. The experimental results showed that higher voltage input yielded higher thermal performance and faster cooling rate. For each set of experiment, the operating frequency and other parameters of the stack were kept unchanged. The experimental results show that the thermal performance and cooling rate increase with the decrease of plate thickness. The largest temperature difference, 14.8°C, was achieved with 0.1 mm plate thickness at voltage setting 9. However, the thermal performance gained for 0.5 mm plate thickness voltage setting of 9, was arguably the optimum thickness in terms of advantages in the ease of fabricating the stack and more consistent cooling.